Antiproliferative Effect of Antimicrobial Hawthorn Crataegus pinntifida Extract Additive Chitosan Hydrogels Against HCT116 Cells and Oxidative Stress Damage Mechanisms

OpenAlex İngilizce

Abstract Oxidative stress can produce high levels of reactive oxygen species following exposure of cells to endogenous and exogenous factors. Recent experiments show that oxidative stress plays an important role in the cytotoxicity of many materials. The aim of this study was to measure the antiproliferative effect of hawthorn Crataegus pinntifida doped with chitosan hydrogels on cultured HCT-116 colon cancer cells and intracellular malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activities. Cell proliferation on hydrogels was examined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay. Cell viability results showed that hydrogels showed significant cytotoxicity activity in HCT116 cells compared to cisplatin. Furthermore, MDA, SOD and CAT activities in HCT-116 colon cancer cells cultured on all hydrogels showed significant differences compared to the control group and each other. The hydrogels showed a significant decrease in MDA levels in HCT116 cells and considerable increase of CAT and SOD activities. According to our findings, we suggest that hawthorn extract hydrogels doped into chitosan may have anticancer activity.